Self-Contained Biological Indicators (BIs) for Steam Sterilization Case of 100 Ensure the efficiency and safety of your steam sterilization processes with our **Self-Contained Biological Indicators (BIs)**, now available in a convenient case of 100. These indicators are designed to meet stringent AAMI standards, providing reliable monitoring for steam sterilizers used in healthcare and laboratory environments. Why Choose Our Self-Contained Biological Indicators? - **Precision Monitoring**: Biologically test steam sterilizers at least weekly and ideally after every load that contains implants to ensure optimal performance and patient safety. - **Easy-to-Follow Instructions**: Each BI comes with clear instructions for recording sterilizer numbers, load numbers, and processing dates, guaranteeing accurate tracking. - **Enhanced Testing Efficiency**: Place one or more BIs in representative loads for testing challenging areas of the sterilizer to confirm effective sterilization. Instructions for Use 1. Record the sterilizer number, load number, and processing date on the BI label. 2. Position the BIs within an instrument tray, rigid container, peel pouch, or similar device as per the representative load. 3. Identify and test the most challenging area in the sterilizer as per its instruction manual (e.g., bottom shelf near the door or center shelf). 4. Follow the sterilizer manufacturer's processing instructions. 5. After processing, ensure the chemical indicator on the label has changed to brown. **Caution**: Allow the BI to cool for ten (10) minutes before crushing to prevent injuries from the pressurized glass ampule. Always wear safety glasses when handling processed BIs. Activation and Incubation 1. Activate the processed BI within 8 hours by gently crushing the inner glass tube. 2. Incubate at **55 ? 60C for 24 hours**, checking periodically for spore growth through a color change from purple to yellow. Note that spore growth can occur as quickly as 2 1/2 hours. Interpreting Test Results 1. Record negative results (no growth) immediately in a Sterilizer Record Notebook; no color change in the media indicates effective sterilization. 2. Report any positive results (evident by color change) to a Supervisor and take the sterilizer out of service until the issue is resolved. Positive growth has been verified for up to 48 hours. 3. For controls, crush an unprocessed BI from the same lot daily during sterilizer testing. Product Features: - **Viable Bacterial Spores**: Each self-contained BI is inoculated with **Geobacillus stearothermophilus** spores, ideal for monitoring saturated steam sterilization at **121C and 132C** in various cycles. - **Versatile Application**: Suitable for different sterilization processes, including gravity displacement and pre-vacuum cycles, with guaranteed effectiveness at **135C for 3 minutes**. - **Regulatory Compliance**: Meets established performance parameters according to current USP and AAMI/ISO 11138 standards for both gravity and dynamic-air-removal sterilization processes. Growth promotion capabilities have been validated for extended steam cycles at **132C for 20 minutes**. Optimize Your Sterilization Process Today! Ensure the highest safety and efficacy in your sterilization procedures with our **self-contained biological indicators**. Shop now to secure your **case of 100** self-contained BIs and maintain compliance with safety standards! --- Keywords: Self-Contained Biological Indicators, Steam Sterilization Monitoring, AAMI Standards, Geobacillus stearothermophilus, Sterilization Test Kit, Biological Indicator for Steam Sterilizers, Healthcare Sterilization Compliance, Sterilizer Performance Verification.